- 移动端
Agrisera AB
7 年
手机商铺
商家活跃：
产品热度：
- NaN
- 0
- 0
- 2
- 2
推荐产品
公司新闻/正文
TBS or TBS-T, how using respective buffer for primary antibody incubation may change the outcome
283 人阅读发布时间：2022-10-20 14:32
| In this experiment, the following conditions were applied: Total protein load/well: 30 µg Blocking: 1 h, 5% non-fat milk Primary antibody: 1:1000 O/N 4°C with TBS-T or TBS buffers Secondary antibody: Goat anti-rabbit, 1:25 000 1hr RT with TBS (AS09 602, Agrisera) Detection: Agrisera ECLSuperBright, 5 min As seen in the image to the righ, using TBS buffer without Tween 20 resulted in the detection of the target protein in the overexpressing line and the wild type. |
|
